Yes. An E-2 spouse is employment-authorized incident to status and may work for the enterprise or any other employer. Her ownership share, if any, also counts toward the Canadian-ownership test if she is a Canadian citizen.
The spouse can be part of the business without a separate application
An E-2 spouse is, under current policy, employment-authorized incident to status, so she may take the office-manager role or work elsewhere without first applying for an employment document. If the couple prefer, she can also hold part of the sixty per cent; as a Canadian citizen her share counts toward the treaty-national ownership requirement, though the develop-and-direct evidence should still centre on whoever actually runs the business. Children under 21 may attend school and may not work, and a child who turns 21 needs a separate basis to remain.
Because E-2 does not lead directly to permanent residence, a Paradise family planning to stay for many years should think about the children's ages early rather than assuming a later fix. The family's status lasts as long as the principal's E-2 and the business's qualification. If the spouse is not a Canadian citizen, her dependant status is unaffected, but her ownership would not count toward the nationality test.
A first review produces an age-and-status calendar for every family member, using passports, marriage records, birth records and anticipated travel dates. Hypothetical example: a violin-restoration shop owner’s child turns 21 during a planned school year; choosing a separate student route early avoids treating the parent’s E-2 renewal as a solution to the child’s age-out.